diff --git a/gga_init.py b/gga_init.py index b77251c406ec4e091f1e98fe63501dc4dcab2cd7..5a7b47d41b45a39e46e39a405eff7ee19ed6ec1f 100644 --- a/gga_init.py +++ b/gga_init.py @@ -11,6 +11,7 @@ import yaml import shutil from pathlib import Path +from jinja2 import Template import utilities import speciesData @@ -19,7 +20,7 @@ import speciesData """ gga_init.py -Usage: $ python3 gga_init.py -i example.yml [OPTIONS] +Usage: $ python3 gga_init.py -i input_example.yml [OPTIONS] """ @@ -56,11 +57,12 @@ class DeploySpeciesStack(speciesData.SpeciesData): # Copy the custom banner to the species dir (banner used in tripal pages) if not self.config["custom_banner"] or not self.config["custom_banner"] == "/path/to/banner" or not self.config["custom_banner"] == "": try: + logging.debug("Custom banner path: %s" % self.config["custom_banner"]) if os.path.isfile(os.path.abspath(self.config["custom_banner"])): shutil.copy(os.path.abspath(self.config["custom_banner"]), "%s/banner.png" % self.species_dir) except FileNotFoundError: - logging.warning("Custom banner (%s), skipping" % self.config["custom_banner"]) + logging.warning("Specified banner not found (%s), skipping" % self.config["custom_banner"]) # Copy nginx conf try: diff --git a/gga_load_data.py b/gga_load_data.py index 945d85d3df23c391d0879b00ce56b51b89d7120e..25b8a65bdcf545ed3c8c67dac96f6f9bfb880cd8 100644 --- a/gga_load_data.py +++ b/gga_load_data.py @@ -23,7 +23,7 @@ from bioblend import galaxy """ gga_load_data.py -Usage: $ python3 gga_init.py -i example.yml [OPTIONS] +Usage: $ python3 gga_init.py -i input_example.yml [OPTIONS] Do not call this script before the galaxy container is ready """ diff --git a/run_workflow_phaeoexplorer.py b/run_workflow_phaeoexplorer.py index 984c00ffded5ce11cf032f10f01b8a481da14cea..c28a769faf002ccc477385e501bba7e92b389391 100644 --- a/run_workflow_phaeoexplorer.py +++ b/run_workflow_phaeoexplorer.py @@ -18,7 +18,7 @@ from bioblend import galaxy """ gga_init.py -Usage: $ python3 gga_init.py -i example.yml [OPTIONS] +Usage: $ python3 gga_init.py -i input_example.yml [OPTIONS] """ @@ -446,86 +446,101 @@ if __name__ == "__main__": else: workflow = os.path.abspath(args.workflow) - # Starting - logging.info("run_workflow.py called for %s" % run_workflow_for_current_organism.full_name) - - # Setting some of the instance attributes - run_workflow_for_current_organism.main_dir = args.main_directory - run_workflow_for_current_organism.species_dir = os.path.join(run_workflow_for_current_organism.main_dir, - run_workflow_for_current_organism.genus_species + - "/") - - # Parse the config yaml file - run_workflow_for_current_organism.config = utilities.parse_config(args.config) - # Set the instance url attribute - for env_variable, value in run_workflow_for_current_organism.config.items(): - if env_variable == "custom_host": - run_workflow_for_current_organism.instance_url = "http://{0}:8888/sp/{1}_{2}/galaxy/".format( - value, run_workflow_for_current_organism.genus_lowercase, run_workflow_for_current_organism.species) - break - else: - run_workflow_for_current_organism.instance_url = "http://localhost:8888/sp/{0}_{1}/galaxy/".format( - run_workflow_for_current_organism.genus_lowercase, - run_workflow_for_current_organism.species) - - run_workflow_for_current_organism.connect_to_instance() - run_workflow_for_current_organism.set_get_history() - # run_workflow_for_current_organism.get_species_history_id() - - # Prepare the instance+history for the current organism (add organism and analyses in Chado) TODO: add argument "setup" - # (althought it should pose no problem as the "Chado add" refuses to duplicate an analysis/organism anyway) - run_workflow_for_current_organism.prepare_history() - - # Get the attributes of the instance and project data files - run_workflow_for_current_organism.get_instance_attributes() - run_workflow_for_current_organism.get_organism_and_analyses_ids() - - # Import datasets into history TODO: put this only for the chado load tripal sync workflow? - run_workflow_for_current_organism.import_datasets_into_history() - - # Explicit workflow parameter names - # TODO: Create distinct methods to call different pre-set workflows using CL arguments/config options (i.e load-chado, jbrowse, functional-annotation, orthology, ...) - - workflow_parameters = dict() - - GENOME_FASTA_FILE = "0" - GFF_FILE = "1" - PROTEINS_FASTA_FILE = "2" - TRANSCRIPTS_FASTA_FILE = "3" - - - LOAD_FASTA_IN_CHADO = "4" - LOAD_GFF_IN_CHADO = "5" - SYNC_ORGANISM_INTO_TRIPAL = "6" - SYNC_GENOME_ANALYSIS_INTO_TRIPAL = "7" - SYNC_OGS_ANALYSIS_INTO_TRIPAL = "8" - SYNC_FEATURES_INTO_TRIPAL = "9" - - workflow_parameters[GENOME_FASTA_FILE] = {} - workflow_parameters[GFF_FILE] = {} - workflow_parameters[PROTEINS_FASTA_FILE] = {} - workflow_parameters[TRANSCRIPTS_FASTA_FILE] = {} - workflow_parameters[LOAD_FASTA_IN_CHADO] = {"organism": run_workflow_for_current_organism.org_id, - "analysis_id": run_workflow_for_current_organism.genome_analysis_id, - "do_update": "true"} - # Change "do_update": "true" to "do_update": "false" in above parameters to prevent appending to the fasta file in chado - # WARNING: It is safer to never update it and just change the genome/ogs versions in the config - workflow_parameters[LOAD_GFF_IN_CHADO] = {"organism": run_workflow_for_current_organism.org_id, - "analysis_id": run_workflow_for_current_organism.ogs_analysis_id} - workflow_parameters[SYNC_ORGANISM_INTO_TRIPAL] = {"organism_id": run_workflow_for_current_organism.org_id} - workflow_parameters[SYNC_GENOME_ANALYSIS_INTO_TRIPAL] = {"analysis_id": run_workflow_for_current_organism.ogs_analysis_id} - workflow_parameters[SYNC_OGS_ANALYSIS_INTO_TRIPAL] = {"analysis_id": run_workflow_for_current_organism.genome_analysis_id} - workflow_parameters[SYNC_FEATURES_INTO_TRIPAL] = {"organism_id": run_workflow_for_current_organism.org_id} - - # Datamap for input datasets - dataset source (type): ldda (LibraryDatasetDatasetAssociation) - run_workflow_for_current_organism.datamap = dict() - run_workflow_for_current_organism.datamap[GENOME_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["genome_file"]} - run_workflow_for_current_organism.datamap[GFF_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["gff_file"]} - run_workflow_for_current_organism.datamap[PROTEINS_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["proteins_file"]} - run_workflow_for_current_organism.datamap[TRANSCRIPTS_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["transcripts_file"]} - - # Run the Chado load Tripal sync workflow with the parameters set above - run_workflow_for_current_organism.run_workflow(workflow_path=workflow, - workflow_parameters=workflow_parameters, - datamap=run_workflow_for_current_organism.datamap, - workflow_name="Chado load Tripal synchronize") + # Verifying the galaxy container is running + if utilities.check_galaxy_state(genus_lowercase=run_workflow_for_current_organism.genus_lowercase, + species=run_workflow_for_current_organism.species, + script_dir=run_workflow_for_current_organism.script_dir): + + # Starting + logging.info("run_workflow.py called for %s" % run_workflow_for_current_organism.full_name) + + # Setting some of the instance attributes + run_workflow_for_current_organism.main_dir = args.main_directory + run_workflow_for_current_organism.species_dir = os.path.join(run_workflow_for_current_organism.main_dir, + run_workflow_for_current_organism.genus_species + + "/") + + # Parse the config yaml file + run_workflow_for_current_organism.config = utilities.parse_config(args.config) + # Set the instance url attribute + for env_variable, value in run_workflow_for_current_organism.config.items(): + if env_variable == "custom_host": + run_workflow_for_current_organism.instance_url = "http://{0}:8888/sp/{1}_{2}/galaxy/".format( + value, run_workflow_for_current_organism.genus_lowercase, run_workflow_for_current_organism.species) + break + else: + run_workflow_for_current_organism.instance_url = "http://localhost:8888/sp/{0}_{1}/galaxy/".format( + run_workflow_for_current_organism.genus_lowercase, + run_workflow_for_current_organism.species) + + run_workflow_for_current_organism.connect_to_instance() + run_workflow_for_current_organism.set_get_history() + # run_workflow_for_current_organism.get_species_history_id() + + # Prepare the instance+history for the current organism (add organism and analyses in Chado) TODO: add argument "setup" + # (althought it should pose no problem as the "Chado add" refuses to duplicate an analysis/organism anyway) + run_workflow_for_current_organism.prepare_history() + + # Get the attributes of the instance and project data files + run_workflow_for_current_organism.get_instance_attributes() + run_workflow_for_current_organism.get_organism_and_analyses_ids() + + # Import datasets into history + # TODO: put this only for the chado load tripal sync workflow? + # TODO: it seems it is not required anymore since using "ldda" option for datasets in the workflow datamap doesn't need the files + run_workflow_for_current_organism.import_datasets_into_history() + + # Explicit workflow parameter names + # TODO: Create distinct methods to call different pre-set workflows using CL arguments/config options (i.e load-chado, jbrowse, functional-annotation, orthology, ...) + + # Chado load and Tripal synchronize workflow + workflow_parameters = {} + + GENOME_FASTA_FILE = "0" + GFF_FILE = "1" + PROTEINS_FASTA_FILE = "2" + TRANSCRIPTS_FASTA_FILE = "3" + + + LOAD_FASTA_IN_CHADO = "4" + LOAD_GFF_IN_CHADO = "5" + SYNC_ORGANISM_INTO_TRIPAL = "6" + SYNC_GENOME_ANALYSIS_INTO_TRIPAL = "7" + SYNC_OGS_ANALYSIS_INTO_TRIPAL = "8" + SYNC_FEATURES_INTO_TRIPAL = "9" + + workflow_parameters[GENOME_FASTA_FILE] = {} + workflow_parameters[GFF_FILE] = {} + workflow_parameters[PROTEINS_FASTA_FILE] = {} + workflow_parameters[TRANSCRIPTS_FASTA_FILE] = {} + workflow_parameters[LOAD_FASTA_IN_CHADO] = {"organism": run_workflow_for_current_organism.org_id, + "analysis_id": run_workflow_for_current_organism.genome_analysis_id, + "do_update": "true"} + # Change "do_update": "true" to "do_update": "false" in above parameters to prevent appending/updates to the fasta file in chado + # WARNING: It is safer to never update it and just change the genome/ogs versions in the config + workflow_parameters[LOAD_GFF_IN_CHADO] = {"organism": run_workflow_for_current_organism.org_id, + "analysis_id": run_workflow_for_current_organism.ogs_analysis_id} + workflow_parameters[SYNC_ORGANISM_INTO_TRIPAL] = {"organism_id": run_workflow_for_current_organism.org_id} + workflow_parameters[SYNC_GENOME_ANALYSIS_INTO_TRIPAL] = {"analysis_id": run_workflow_for_current_organism.ogs_analysis_id} + workflow_parameters[SYNC_OGS_ANALYSIS_INTO_TRIPAL] = {"analysis_id": run_workflow_for_current_organism.genome_analysis_id} + workflow_parameters[SYNC_FEATURES_INTO_TRIPAL] = {"organism_id": run_workflow_for_current_organism.org_id} + + # Datamap for input datasets - dataset source (type): ldda (LibraryDatasetDatasetAssociation) + run_workflow_for_current_organism.datamap = {} + run_workflow_for_current_organism.datamap[GENOME_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["genome_file"]} + run_workflow_for_current_organism.datamap[GFF_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["gff_file"]} + run_workflow_for_current_organism.datamap[PROTEINS_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["proteins_file"]} + run_workflow_for_current_organism.datamap[TRANSCRIPTS_FASTA_FILE] = {"src": "ldda", "id": run_workflow_for_current_organism.datasets["transcripts_file"]} + + # Run the Chado load Tripal sync workflow with the parameters set above + run_workflow_for_current_organism.run_workflow(workflow_path=workflow, + workflow_parameters=workflow_parameters, + datamap=run_workflow_for_current_organism.datamap, + workflow_name="Chado load Tripal synchronize") + + # Jbrowse creation workflow + + workflow_parameters = {} + + else: + logging.critical("The galaxy container for %s is not ready yet!" % run_workflow_for_current_organism.full_name) diff --git a/workflows/Chado_load_Tripal_synchronize.ga b/workflows/Chado_load_Tripal_synchronize.ga index a194b03268c48c4300a3b2a0f6b759cbf0fb6c6b..c8ce7a4549828092413ddc6a4c0e2ca033ea44f8 100644 --- a/workflows/Chado_load_Tripal_synchronize.ga +++ b/workflows/Chado_load_Tripal_synchronize.ga @@ -16,14 +16,20 @@ "outputs": [], "position": { "left": 200, - "top": 200 + "top": 227 }, "tool_id": null, "tool_state": "{\"optional\": false}", "tool_version": null, "type": "data_input", "uuid": "89e7487e-004d-4db1-b5eb-1676b98aebde", - "workflow_outputs": [] + "workflow_outputs": [ + { + "label": null, + "output_name": "output", + "uuid": "08f77326-1435-4fad-a610-e56f06a0ced5" + } + ] }, "1": { "annotation": "", @@ -37,14 +43,20 @@ "outputs": [], "position": { "left": 200, - "top": 290 + "top": 317 }, "tool_id": null, "tool_state": "{\"optional\": false}", "tool_version": null, "type": "data_input", "uuid": "1d25f54c-7575-4c8d-be55-73dd7e58613f", - "workflow_outputs": [] + "workflow_outputs": [ + { + "label": null, + "output_name": "output", + "uuid": "e8f16746-c91f-4147-b460-f1052650e699" + } + ] }, "2": { "annotation": "", @@ -58,14 +70,20 @@ "outputs": [], "position": { "left": 200, - "top": 380 + "top": 407 }, "tool_id": null, "tool_state": "{\"optional\": false}", "tool_version": null, "type": "data_input", "uuid": "6c1a20fa-828a-404c-b107-76fb8ddf3954", - "workflow_outputs": [] + "workflow_outputs": [ + { + "label": null, + "output_name": "output", + "uuid": "98ade546-95a4-477c-bd19-38d278849b98" + } + ] }, "3": { "annotation": "", @@ -79,38 +97,44 @@ "outputs": [], "position": { "left": 200, - "top": 470 + "top": 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"annotation": "", - "content_id": "toolshed.g2.bx.psu.edu/repos/gga/chado_feature_load_gff/feature_load_gff/2.3.4+galaxy0", + "content_id": "toolshed.g2.bx.psu.edu/repos/gga/chado_feature_load_gff/feature_load_gff/2.3.3", "errors": null, "id": 5, "input_connections": { @@ -165,6 +189,10 @@ } }, "inputs": [ + { + "description": "runtime parameter for tool Chado load gff", + "name": "analysis_id" + }, { "description": "runtime parameter for tool Chado load gff", "name": "fasta" @@ -173,6 +201,10 @@ "description": "runtime parameter for tool Chado load gff", "name": "gff" }, + { + "description": "runtime parameter for tool Chado load gff", + "name": "organism" + }, { "description": "runtime parameter for tool Chado load gff", "name": "wait_for" @@ -188,25 +220,25 @@ ], "position": { "left": 772, - "top": 200 + "top": 227 }, "post_job_actions": {}, - "tool_id": "toolshed.g2.bx.psu.edu/repos/gga/chado_feature_load_gff/feature_load_gff/2.3.4+galaxy0", + "tool_id": 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