diff --git a/gga_get_data.py b/gga_get_data.py index d57f0fb51fafcc374c9394834f602ded41d0aafe..20ec80d37f1b1449fd216ae616d8f594976e952a 100755 --- a/gga_get_data.py +++ b/gga_get_data.py @@ -122,20 +122,14 @@ class GetData(speciesData.SpeciesData): # Where to store blast results? # search_excluded_datasets = ["interpro_path", "orthofinder_path", "blastp_path", "blastx_path"] - # # These datasets will not be searched if missing in the input file - - dataset_shortname = "" - if self.sex is not None or self.sex != "": - dataset_shortname = self.genus[0].lower() + "_" + self.species.lower() + "_" + self.sex[0].lower() - else: - dataset_shortname = self.genus[0].lower() + "_" + self.species.lower() + # These datasets will not be searched if missing in the input file # Copy datasets in the organism src_data dir tree correct folder for k, v in datasets_to_get.items(): if v: # If dataset is not present in input file, skip copy if k in genome_datasets: logging.info("Copying {0} ({1}) into {2}".format(k, v, organism_genome_dir)) - genome_fname = "{0}_v{1}.fasta".format(dataset_shortname, self.genome_version) + genome_fname = "{0}_v{1}.fasta".format(self.dataset_prefix, self.genome_version) try: shutil.copyfile(os.path.abspath(v), os.path.join(organism_genome_dir, genome_fname)) except Exception as exc: @@ -143,19 +137,19 @@ class GetData(speciesData.SpeciesData): elif k in annotation_datasets: dataset_fname = "" if k == "gff_path": - dataset_fname = "{0}_OGS{1}.gff".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}.gff".format(self.dataset_prefix, self.ogs_version) elif k == "transcripts_path": - dataset_fname = "{0}_OGS{1}_transcripts.fasta".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_transcripts.fasta".format(self.dataset_prefix, self.ogs_version) elif k == "proteins_path": - dataset_fname = "{0}_OGS{1}_proteins.fasta".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_proteins.fasta".format(self.dataset_prefix, self.ogs_version) elif k == "orthofinder_path": - dataset_fname = "{0}_OGS{1}_orthofinder.tsv".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_orthofinder.tsv".format(self.dataset_prefix, self.ogs_version) elif k == "interpro_path": - dataset_fname = "{0}_OGS{1}_interproscan.xml".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_interproscan.xml".format(self.dataset_prefix, self.ogs_version) elif k == "blastp_path": - dataset_fname = "{0}_OGS{1}_blastp.xml".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_blastp.xml".format(self.dataset_prefix, self.ogs_version) elif k == "blastx_path": - dataset_fname = "{0}_OGS{1}_blastx.xml".format(dataset_shortname, self.genome_version) + dataset_fname = "{0}_OGS{1}_blastx.xml".format(self.dataset_prefix, self.ogs_version) logging.info("Copying {0} ({1}) into {2}".format(k, v, organism_annotation_dir)) try: shutil.copyfile(os.path.abspath(v), os.path.join(organism_annotation_dir, dataset_fname)) diff --git a/gga_init.py b/gga_init.py index fbefcebd1c2ce145db1270455cb8518ce3dd47a9..bd1beb4e7ebed69a7945fb0e8ed58a4545bf3483 100755 --- a/gga_init.py +++ b/gga_init.py @@ -64,8 +64,9 @@ class DeploySpeciesStack(speciesData.SpeciesData): if not config["banner_path"] == "" and os.path.isfile(os.path.abspath(config["banner_path"])): banner_dest_path = os.path.abspath("./banner.png") logging.info("Custom banner path: %s" % self.config["banner_path"]) - if os.path.samefile(os.path.abspath(config["banner_path"]),banner_dest_path): - shutil.copy(os.path.abspath(self.config["banner_path"]), banner_dest_path) + # if os.path.samefile(os.path.abspath(config["banner_path"]), banner_dest_path): + # shutil.copy(os.path.abspath(self.config["banner_path"]), banner_dest_path) + shutil.copy(os.path.abspath(self.config["banner_path"]), banner_dest_path) else: logging.debug("Using default banner for Tripal pages") self.config.pop("banner_path", None) diff --git a/gga_load_data.py b/gga_load_data.py index cc73e3e2b6dcc0b24dc46503366a15fde4f0a951..c4ed5594a9d3d173bd11d4ee4918a1152cd6a5ca 100755 --- a/gga_load_data.py +++ b/gga_load_data.py @@ -64,13 +64,13 @@ class LoadData(speciesData.SpeciesData): try: histories = self.instance.histories.get_histories(name=str(self.genus_species)) self.history_id = histories[0]["id"] - logging.debug("History ID set for {0}: {1}".format(self.full_name, self.history_id)) + logging.debug("History ID set for {0} {1}: {2}".format(self.genus, self.species, self.history_id)) except IndexError: - logging.info("Creating history for %s" % self.full_name) + logging.info("Creating history for {0} {1}".format(self.genus, self.species)) self.instance.histories.create_history(name=str(self.genus_species)) histories = self.instance.histories.get_histories(name=str(self.genus_species)) self.history_id = histories[0]["id"] - logging.debug("History ID set for {0}: {1}".format(self.full_name, self.history_id)) + logging.debug("History ID set for {0} {1}: {2}".format(self.genus, self.species, self.history_id)) return self.history_id @@ -391,9 +391,9 @@ if __name__ == "__main__": load_data_for_current_species.connect_to_instance() # Load the datasets into a galaxy library - logging.info("Setting up library for %s" % load_data_for_current_species.full_name) + logging.info("Setting up library for {0} {1}".format(load_data_for_current_species.genus, load_data_for_current_species.species)) load_data_for_current_species.setup_library() - logging.info("Successfully set up library in galaxy for %s" % load_data_for_current_species.full_name) + logging.info("Successfully set up library in galaxy for {0} {1}".format(load_data_for_current_species.genus, load_data_for_current_species.species)) # Set or get the history for the current organism load_data_for_current_species.set_get_history() @@ -407,8 +407,8 @@ if __name__ == "__main__": # load_data_for_current_species.purge_histories() # Testing purposes - logging.info("Data successfully loaded and imported for %s" % load_data_for_current_species.full_name) + logging.info("Data successfully loaded and imported for {0} {1}".format(load_data_for_current_species.genus, load_data_for_current_species.species)) else: - logging.critical("The galaxy container for %s is not ready yet!" % load_data_for_current_species.full_name) + logging.critical("The galaxy container for {0} {1} is not ready yet".format(load_data_for_current_species.genus, load_data_for_current_species.species)) sys.exit() diff --git a/run_workflow_phaeoexplorer.py b/run_workflow_phaeoexplorer.py index 7f273a6b50094bdcd79a9fbd0c8deec8c60b25d6..2c385d476ca128e949e9b9a1353c0679ed5d6958 100755 --- a/run_workflow_phaeoexplorer.py +++ b/run_workflow_phaeoexplorer.py @@ -252,7 +252,6 @@ class RunWorkflow(speciesData.SpeciesData): tool_version = "2.3.4+galaxy0" - get_organism_tool = self.instance.tools.show_tool("toolshed.g2.bx.psu.edu/repos/gga/chado_organism_get_organisms/organism_get_organisms/2.3.4+galaxy0") get_organisms = self.instance.tools.run_tool( @@ -564,32 +563,24 @@ class RunWorkflow(speciesData.SpeciesData): self.datasets["blast_diamond_file"] = e["ldda_id"] self.datasets_name["blast_diamond_file"] = e["name"] - dataset_shortname = "" - if self.sex is not None or self.sex != "": - dataset_shortname = self.genus[0].lower() + "_" + self.species.lower() + "_" + self.sex[0].lower() - else: - dataset_shortname = self.genus[0].lower() + "_" + self.species.lower() history_datasets_li = self.instance.datasets.get_datasets() genome_hda_id, gff_hda_id, transcripts_hda_id, proteins_hda_id, blast_diamond_hda_id, interproscan_hda_id = None, None, None, None, None, None - # Finding datasets in history (matches datasets name) - # TODO: match version as well - # TODO: match whoole dataset name (not just prefix and affix) + # Finding datasets in history (matching datasets names) for dataset in history_datasets_li: dataset_name = dataset["name"] - if dataset_shortname in dataset_name: - dataset_id = dataset["id"] - if dataset_name.endswith("%s.fasta" % self.genome_version): - genome_hda_id = dataset_id - if dataset_name.endswith(".gff"): - gff_hda_id = dataset_id - if dataset_name.endswith("transcripts.fasta"): - transcripts_hda_id = dataset_id - if dataset_name.endswith("proteins.fasta"): - proteins_hda_id = dataset_id - if dataset_name.endswith("blasp.xml"): - blast_diamond_hda_id = dataset_id + dataset_id = dataset["id"] + if dataset_name == "{0}_v{1}.fasta".format(self.dataset_prefix, self.genome_version): + genome_hda_id = dataset_id + if dataset_name == "{0}_OGS{1}.gff".format(self.dataset_prefix, self.ogs_version): + gff_hda_id = dataset_id + if dataset_name == "{0}_OGS{1}_transcripts.fasta".format(self.dataset_prefix, self.ogs_version): + transcripts_hda_id = dataset_id + if dataset_name == "{0}_OGS{1}_proteins.fasta".format(self.dataset_prefix, self.ogs_version): + proteins_hda_id = dataset_id + if dataset_name == "{0}_OGS{1}_blastx.xml".format(self.dataset_prefix, self.ogs_version): + blast_diamond_hda_id = dataset_id # Import each dataset into history if it is not imported @@ -620,6 +611,14 @@ class RunWorkflow(speciesData.SpeciesData): except Exception as exc: logging.debug("Blastp file not found in library (history: {0})".format(self.history_id)) + logging.debug("History dataset IDs (hda_id) for %s:" % self.full_name) + logging.debug({"genome_hda_id": genome_hda_id, + "gff_hda_id": gff_hda_id, + "transcripts_hda_id": transcripts_hda_id, + "proteins_hda_id": proteins_hda_id, + "blast_diamond_hda_id": blast_diamond_hda_id, + "interproscan_hda_id": interproscan_hda_id}) + # Return a dict made of the hda ids return {"genome_hda_id": genome_hda_id, "gff_hda_id": gff_hda_id, @@ -987,7 +986,7 @@ if __name__ == "__main__": logging.info("Input organism %s: 2 species detected in input dictionary" % k) # Set workflow path (2 organisms) - workflow_path = os.path.join(os.path.abspath(script_dir), "workflows_phaeoexplorer/Galaxy-Workflow-chado_load_tripal_synchronize_jbrowse_2org_v2.ga") + workflow_path = os.path.join(os.path.abspath(script_dir), "workflows_phaeoexplorer/Galaxy-Workflow-chado_load_tripal_synchronize_jbrowse_2org_v4.ga") # Instance object required variables instance_url, email, password = None, None, None @@ -1038,6 +1037,7 @@ if __name__ == "__main__": "org1_org_id": org1_org_id, "org1_genome_analysis_id": org1_genome_analysis_id, "org1_ogs_analysis_id": org1_ogs_analysis_id, + "org1_genome_hda_id": org1_genome_hda_id, "org1_transcripts_hda_id": org1_transcripts_hda_id, "org1_proteins_hda_id": org1_proteins_hda_id, "org1_gff_hda_id": org1_gff_hda_id, @@ -1046,7 +1046,7 @@ if __name__ == "__main__": # Look for empty parameters values, throw a critical error if a parameter value is invalid for param_name, param_value in org1_parameters_dict.items(): if param_value is None or param_value == "": - logging.critical("Empty parameter found for organism {0} (parameter: {1}, parameter value: {2})".format(org1_full_name, param_name, param_value)) + logging.critical("Empty parameter value found for organism {0} (parameter: {1}, parameter value: {2})".format(org1_full_name, param_name, param_value)) sys.exit() # Organism 2 attributes @@ -1077,6 +1077,7 @@ if __name__ == "__main__": "org2_org_id": org2_org_id, "org2_genome_analysis_id": org2_genome_analysis_id, "org2_ogs_analysis_id": org2_ogs_analysis_id, + "org2_genome_hda_id": org2_genome_hda_id, "org2_transcripts_hda_id": org2_transcripts_hda_id, "org2_proteins_hda_id": org2_proteins_hda_id, "org2_gff_hda_id": org2_gff_hda_id, @@ -1085,12 +1086,24 @@ if __name__ == "__main__": # Look for empty parameters values, throw a critical error if a parameter value is invalid for param_name, param_value in org2_parameters_dict.items(): if param_value is None or param_value == "": - logging.critical("Empty parameter found for organism {0} (parameter: {1}, parameter value: {2})".format(org2_full_name, param_name, param_value)) + logging.critical("Empty parameter value found for organism {0} (parameter: {1}, parameter value: {2})".format(org2_full_name, param_name, param_value)) sys.exit() + + jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="\{id\}") + jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + if "menu_url" not in config.keys(): + jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="\{id\}") + jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + else: + jbrowse_menu_url_org1 = config["menu_url"] + jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + + # Source files association (ordered by their IDs in the workflow) - GENOME_FASTA_FILE_ORG1 = "0" - GFF_FILE_ORG1 = "1" + # WARNING: Be very careful about how the workflow is "organized" (i.e the order of the steps/datasets, check the .ga if there is any error) + GFF_FILE_ORG1 = "0" + GENOME_FASTA_FILE_ORG1 = "1" PROTEINS_FASTA_FILE_ORG1 = "2" GENOME_FASTA_FILE_ORG2 = "3" @@ -1127,17 +1140,19 @@ if __name__ == "__main__": workflow_parameters[LOAD_FASTA_ORG1] = {"organism": org1_org_id, "analysis_id": org1_genome_analysis_id, "do_update": "true"} - workflow_parameters[JBROWSE_ORG1] = {} + # workflow_parameters[JBROWSE_ORG1] = {"menu_url": jbrowse_menu_url_org1} + workflow_parameters[JBROWSE_ORG1] = {} workflow_parameters[LOAD_GFF_ORG1] = {"organism": org1_org_id, "analysis_id": org1_ogs_analysis_id} workflow_parameters[SYNC_FEATURES_ORG1] = {"organism_id": org1_org_id} + # workflow_parameters[JBROWSE_CONTAINER] = {"organisms": [{"name": org1_full_name, "unique_id": org1_species_folder_name, }, {"name": org2_full_name, "unique_id": org2_species_folder_name}]} workflow_parameters[JBROWSE_CONTAINER] = {} - # Organism 2 workflow_parameters[LOAD_FASTA_ORG2] = {"organism": org2_org_id, "analysis_id": org2_genome_analysis_id, "do_update": "true"} workflow_parameters[LOAD_GFF_ORG2] = {"organism": org2_org_id, "analysis_id": org2_ogs_analysis_id} + # workflow_parameters[JRBOWSE_ORG2] = {"menu_url": jbrowse_menu_url_org2} workflow_parameters[JRBOWSE_ORG2] = {} workflow_parameters[SYNC_FEATURES_ORG2] = {"organism_id": org2_org_id} @@ -1166,26 +1181,33 @@ if __name__ == "__main__": workflow_dict = json.load(ga_in_file) workflow_name = workflow_dict["name"] + # For the Jbrowse tool, we unfortunately have to manually edit the parameters instead of setting them # as runtime values, using runtime parameters makes the tool throw an internal critical error ("replace not found" error) # Scratchgmod test: need "http" (or "https"), the hostname (+ port) - jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="\{id\}") - jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="{id}") + jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="{id}") if "menu_url" not in config.keys(): - jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="\{id\}") - jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + jbrowse_menu_url_org1 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="{id}") + jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="{id}") else: - jbrowse_menu_url_org1 = config["menu_url"] - jbrowse_menu_url_org2 = "https://{hostname}/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(hostname=config["hostname"], genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="\{id\}") + jbrowse_menu_url_org1 = config["menu_url"] + "/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(genus_sp=org1_genus_species, Genus=org1_genus[0].upper() + org1_genus[1:], species=org1_species, id="{id}") + jbrowse_menu_url_org2 = config["menu_url"] + "/sp/{genus_sp}/feature/{Genus}/{species}/mRNA/{id}".format(genus_sp=org2_genus_species, Genus=org2_genus[0].upper() + org2_genus[1:], species=org2_species, id="{id}") + # show_tool_add_organism = instance.tools.show_tool(tool_id="toolshed.g2.bx.psu.edu/repos/gga/chado_organism_add_organism/organism_add_organism/2.3.4+galaxy0", io_details=True) + # print(show_tool_add_organism) + # show_jbrowse_tool = instance.tools.show_tool(tool_id="toolshed.g2.bx.psu.edu/repos/iuc/jbrowse/jbrowse/1.16.11+galaxy0", io_details=True) + # print(show_jbrowse_tool) + # show_jbrowse_container_tool = instance.tools.show_tool(tool_id="toolshed.g2.bx.psu.edu/repos/gga/jbrowse_to_container/jbrowse_to_container/0.5.1", io_details=True) + # print(show_jbrowse_container_tool) # Replace values in the workflow dictionary workflow_dict["steps"]["7"]["tool_state"] = workflow_dict["steps"]["7"]["tool_state"].replace("__MENU_URL_ORG1__", jbrowse_menu_url_org1) workflow_dict["steps"]["8"]["tool_state"] = workflow_dict["steps"]["8"]["tool_state"].replace("__MENU_URL_ORG2__", jbrowse_menu_url_org2) # The UNIQUE_ID is specific to a combination genus_species_strain_sex so every combination should have its unique workflow # in galaxy --> define a naming method for these workflows - workflow_dict["steps"]["10"]["tool_state"] = workflow_dict["steps"]["10"]["tool_state"].replace("__FULL_NAME_ORG1__", org1_full_name).replace("__UNIQUE_ID_ORG1__", org1_species_folder_name) - workflow_dict["steps"]["10"]["tool_state"] = workflow_dict["steps"]["10"]["tool_state"].replace("__FULL_NAME_ORG2__", org2_full_name).replace("__UNIQUE_ID_ORG2__", org2_species_folder_name) + workflow_dict["steps"]["10"]["tool_state"] = workflow_dict["steps"]["10"]["tool_state"].replace("__DISPLAY_NAME_ORG1__", org1_full_name).replace("__UNIQUE_ID_ORG1__", org1_species_folder_name) + workflow_dict["steps"]["10"]["tool_state"] = workflow_dict["steps"]["10"]["tool_state"].replace("__DISPLAY_NAME_ORG2__", org2_full_name).replace("__UNIQUE_ID_ORG2__", org2_species_folder_name) # Import the workflow in galaxy as a dict instance.workflows.import_workflow_dict(workflow_dict=workflow_dict) diff --git a/speciesData.py b/speciesData.py index de246d7eb798f7ca1d36b90ba6b159fae7fe8765..a8637b01484de65a257f12eec6e5afacc406d014 100755 --- a/speciesData.py +++ b/speciesData.py @@ -67,6 +67,11 @@ class SpeciesData: self.abbreviation = "_".join(utilities.filter_empty_not_empty_items([self.genus_lowercase[0], self.species, self.strain, self.sex])["not_empty"]) self.genus_species = "{0}_{1}".format(self.genus.lower(), self.species.lower()) + self.dataset_prefix = None + if self.sex is not None or self.sex != "": + self.dataset_prefix = self.genus[0].lower() + "_" + self.species.lower() + "_" + self.sex[0].lower() + else: + self.dataset_prefix = self.genus[0].lower() + "_" + self.species.lower() # Bioblend/Chado IDs for an organism analyses/organisms/datasets/history/library self.org_id = None diff --git a/workflows_phaeoexplorer/Galaxy-Workflow-chado_load_tripal_synchronize_jbrowse_2org_v4.ga b/workflows_phaeoexplorer/Galaxy-Workflow-chado_load_tripal_synchronize_jbrowse_2org_v4.ga new file mode 100644 index 0000000000000000000000000000000000000000..045e442219face7eed174cafcec206dd9de0ffa0 --- /dev/null +++ b/workflows_phaeoexplorer/Galaxy-Workflow-chado_load_tripal_synchronize_jbrowse_2org_v4.ga @@ -0,0 +1,881 @@ +{ + "a_galaxy_workflow": "true", + "annotation": "", + "format-version": "0.1", + "name": "chado_load_tripal_synchronize_jbrowse_2org_v4", + "steps": { + "0": { + "annotation": "", + "content_id": null, + "errors": null, + "id": 0, + "input_connections": {}, + "inputs": [ + { + "description": "", + "name": 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