diff --git a/gga_preprocessing_phaeoexplorer.py b/gga_preprocessing_phaeoexplorer.py
deleted file mode 100644
index 886ec180f8154cc4fb7b5d65e8e004567c5cd040..0000000000000000000000000000000000000000
--- a/gga_preprocessing_phaeoexplorer.py
+++ /dev/null
@@ -1,313 +0,0 @@
-#!/usr/bin/env python3
-# -*- coding: utf-8 -*-
-
-import bioblend
-import argparse
-import os
-import subprocess
-import logging
-import sys
-import fnmatch
-import time
-import json
-import re
-import stat
-import shutil
-import tarfile
-
-import utilities
-import speciesData
-
-# Import the phaeoexplorer-tools gga_preprocessing modules (descriptions transfer for the gff file)
-sys.path.append("/projet/sbr/gga/dev/gga/swarm/gga/swarm/phaeoexplorer-tools/gga_preprocessing") # Change this path (path to module) in production
-from gga_preprocessing import phaeoexplorer_hectar_transfer
-from gga_preprocessing import ec_32_orthologs_transfer
-
-"""
-gga_preprocessing_phaeoexplorer.py
-
-Usage: $ python3 gga_preprocessing_phaeoexplorer.py -i input_example.yml --config config.yml [OPTIONS]
-
-
-This script is streamlined with "gga_get_data.py" from the "gga_load_data" repo and cannot be used separately
-
-"""
-
-
-class GgaPreprocess(speciesData.SpeciesData):
- """
- Child of SpeciesData
-
- Methods to process datasets and results from Phaeoexplorer for the gga pipeline
-
- """
-
- def goto_species_dir(self):
- """
- Go to the species directory (starting from the main dir)
-
- :return:
- """
-
- os.chdir(self.main_dir)
- species_dir = os.path.join(self.main_dir, self.genus_species) + "/"
- try:
- os.chdir(species_dir)
- except OSError:
- logging.critical("Cannot access %s" % species_dir)
- sys.exit(0)
- return 1
-
-
- def transfer_manual_annotations(self):
- """
- Transfer manual annotations to the species gff file
-
- :return:
- """
- self.goto_species_dir()
-
- # Location of the manual annotation file (tabulated)
- manual_annotation_file = "/projet/sbr/phaeoexplorer_raw/finalresult/functional_annotation_orthofinder/EctsiV2_gene_info_LATEST.txt"
-
- if not self.sex or self.sex == "" or self.sex is None:
- orthofinder_file = "/projet/sbr/phaeoexplorer_raw/finalresult/functional_annotation_orthofinder/ORTHOLOGUES_EC32/{0}-{1}_proteins__v__Ectocarpus-sp_Ec32_proteins-ISOFORM1.tsv".format(self.genus_uppercase, self.species)
- else:
- orthofinder_file = "/projet/sbr/phaeoexplorer_raw/finalresult/functional_annotation_orthofinder/ORTHOLOGUES_EC32/{0}-{1}_{2}_proteins__v__Ectocarpus-sp_Ec32_proteins-ISOFORM1.tsv".format(self.genus_uppercase, self.species, self.sex.upper())
-
- if os.path.exists(manual_annotation_file):
- if os.path.isfile(orthofinder_file):
- # file_basename = os.path.basename(orthofinder_file)
- for d in [i[0] for i in os.walk(os.getcwd() + "/src_data")]:
- if "annotation" in d and self.species_folder_name in d and self.ogs_version in d:
- for f in os.listdir(d):
- if f.endswith(".gff"):
- gff_file = os.path.join(d, f)
- annotation_dir = os.path.abspath(d)
- # shutil.copy(orthofinder_file, os.path.join(d, file_basename))
- if os.path.isfile(manual_annotation_file) and os.path.isfile(gff_file) and os.path.isfile(orthofinder_file):
- logging.info("Transferring manual annotations from {0} to {1}".format(manual_annotation_file, gff_file))
- manual_annotations_transfer.orthologs_transfer(manual_annotation_file=manual_annotation_file,
- orthofinder_file=orthofinder_file,
- gff_file=gff_file,
- outdir=annotation_dir)
- new_gff_file = os.path.join(d, "%s_TRANSFERRED_MANUAL.gff" % os.path.basename(gff_file))
- # if not os.path.isfile(new_gff_file):
- # new_gff_file = os.path.join(d, "{0}-{1}_TRANSFERRED_MANUAL.gff".format(self.genus_uppercase, self.species))
- os.replace(os.path.abspath(new_gff_file), os.path.abspath(gff_file))
-
- # os.replace()
-
- else:
- logging.info("Orthofinder output file ({0}) doesn't exist for {1}".format(orthofinder_file, self.full_name))
- else:
- logging.info("Manual annotation file ({0}) doesn't exist for {1}".format(manual_annotation_file, self.full_name))
-
-
-
- return 1
-
-
- def transfer_hectar_annotations(self):
- """
- Extract hectar output file and transfer hectar annotations to the species gff file
-
- The extracted Hectar output file is deleted upon completion
-
- :return:
- """
- self.goto_species_dir()
-
- hectar_output_archive = "/projet/sbr/phaeoexplorer_raw/finalresult/functional_annotation_hectar/finalresult_hectar_phaeoexplorer_20200228.tgz"
- # Hectar archive containing all hectar outputs (path might change)
-
- if os.path.exists(hectar_output_archive):
- for d in [i[0] for i in os.walk(os.getcwd() + "/src_data")]:
- if "annotation" in d and self.species_folder_name in d and self.ogs_version in d:
- for f in os.listdir(d):
- if f.endswith(".gff"):
- gff_file = os.path.join(d, f)
- annotation_dir = os.path.abspath(d)
- hectar_file = ""
- logging.info("Extracting hectar output file for %s" % self.full_name)
- with tarfile.open(os.path.abspath(hectar_output_archive)) as archive_tar:
- if not self.sex or self.sex == "" or self.sex is None:
- for archive_file in archive_tar.getmembers():
- archive_tar.extract("finalresult/{0}-{1}/hectar_output.csv".format(self.genus_uppercase, self.species),
- path=self.annotation_dir)
- hectar_file = os.path.join(annotation_dir, "finalresult/{0}-{1}/hectar_output.csv".format(self.genus_uppercase, self.species))
- else:
- for archive_file in archive_tar.getmembers():
- archive_tar.extract("finalresult/{0}-{1}_{2}/hectar_output.csv".format(self.genus_uppercase, self.species, self.sex.upper()),
- path=annotation_dir)
- hectar_file = os.path.join(annotation_dir, "finalresult/{0}-{1}_{2}/hectar_output.csv".format(self.genus_uppercase, self.species, self.sex.upper()))
-
- if os.path.isfile(hectar_file):
- hectar_transfer.hectar_transfer(hectar_file=hectar_file,
- gff_file=gff_file,
- outdir=annotation_dir)
- new_gff_file = os.path.join(d, "%s_TRANSFERRED_HECTAR.gff" % os.path.basename(gff_file))
- # if not os.path.isfile(new_gff_file):
- # new_gff_file = os.path.join(d, "{0}-{1}_TRANSFERRED_HECTAR.gff".format(self.genus_uppercase, self.species))
- os.replace(os.path.abspath(new_gff_file), os.path.abspath(gff_file))
- else:
- logging.info("Hectar output archive ({0}) doesn't exist for {1}".format(hectar_output_archive, self.full_name))
-
- return 1
-
-
-
- def batch_modify_fasta_headers(self):
- """
- Change the fasta headers before integration, so that the indexing tool in galaxy interprets the headers
- correctly and doesn't throw an error
-
- The function will use the class attribute "source_datasets", pointing to files in the galaxy
- library to find the fasta files that need their headers formatted
-
- :return:
- """
-
- proteins_file = None
- proteins_outfile = None
- annotation_dir = None
- organism_annotation_dir = os.path.abspath("./src_data/annotation/{0}/OGS{1}".format(self.species_folder_name, self.ogs_version))
-
- self.goto_species_dir()
-
- for d in [i[0] for i in os.walk(os.getcwd() + "/src_data")]:
- if "annotation" in d and self.species_folder_name in d and self.ogs_version in d:
- for f in os.listdir(d):
- if "proteins" in f:
- proteins_file = os.path.join(d, f)
- proteins_outfile = os.path.join(d, "outfile_proteins.fa")
- annotation_dir = os.path.abspath(d)
- # Formatting the headers
- if proteins_file is not None:
- self.format_fasta_headers(infile=proteins_file,
- outfile=proteins_outfile,
- pattern="^>mRNA",
- repl=">protein")
- if os.path.exists(annotation_dir + "/outfile_proteins.fa"):
- subprocess.call(["mv", annotation_dir + "/outfile_proteins.fa", proteins_file],
- stdout=subprocess.PIPE,
- stderr=subprocess.PIPE,
- cwd=annotation_dir)
- subprocess.call(["rm", annotation_dir + "/outfile_proteins.fa"], stdout=subprocess.PIPE, stderr=subprocess.PIPE, cwd=annotation_dir)
-
- else:
- logging.warning("Skipping proteins fasta headers formatting (FileNotFound)")
-
- @staticmethod
- def format_fasta_headers(infile, outfile, pattern, repl):
- """
- Format the fasta headers of a given file, given a matching pattern and a replacement string
-
- :param infile:
- :param outfile:
- :param pattern:
- :param repl:
- :return:
- """
-
- infile = open(infile, 'r')
- outfile = open(outfile, 'w')
-
- lines = infile.readlines()
-
- for line in lines:
- line_out = re.sub(pattern, repl, line)
- outfile.write(line_out)
-
- infile.close()
- outfile.close()
-
-
- def clean_up_dir_tree(self):
- """
-
- :return:
- """
-
- organism_annotation_dir = os.path.abspath("./src_data/annotation/{0}/OGS{1}".format(self.species_folder_name, self.ogs_version))
- if os.path.exists(os.path.join(organism_annotation_dir, "finalresult")):
- shutil.rmtree(os.path.join(organism_annotation_dir, "finalresult"))
-
- return 1
-
-
-if __name__ == "__main__":
- parser = argparse.ArgumentParser(description="Automatic data loading in containers and interaction "
- "with galaxy instances for GGA"
- ", following the protocol @ "
- "http://gitlab.sb-roscoff.fr/abims/e-infra/gga")
-
- parser.add_argument("input",
- type=str,
- help="Input file (yml)")
-
- parser.add_argument("-v", "--verbose",
- help="Increase output verbosity",
- action="store_false")
-
- parser.add_argument("--config",
- type=str,
- help="Config path, default to the 'config' file inside the script repository")
-
- parser.add_argument("--main-directory",
- type=str,
- help="Where the stack containers will be located, defaults to working directory")
-
- args = parser.parse_args()
-
- if args.verbose:
- logging.basicConfig(level=logging.DEBUG)
- else:
- logging.basicConfig(level=logging.INFO)
- logging.getLogger("urllib3").setLevel(logging.WARNING)
-
- # Parsing the config file if provided, using the default config otherwise
- if not args.config:
- args.config = os.path.join(os.path.dirname(os.path.realpath(sys.argv[0])), "config")
- else:
- args.config = os.path.abspath(args.config)
-
- if not args.main_directory:
- args.main_directory = os.getcwd()
- else:
- args.main_directory = os.path.abspath(args.main_directory)
-
- sp_dict_list = utilities.parse_input(args.input)
-
- for sp_dict in sp_dict_list:
-
- # Creating an instance of gga_preprocessor object
- gga_preprocessor = GgaPreprocess(parameters_dictionary=sp_dict)
-
- # Starting
- logging.info("gga_preprocessing_phaeoexplorer.py called for %s" % gga_preprocessor.full_name)
-
- # Setting some of the instance attributes
- gga_preprocessor.main_dir = args.main_directory
- gga_preprocessor.species_dir = os.path.join(gga_preprocessor.main_dir,
- gga_preprocessor.genus_species +
- "/")
-
- # Parse the config yaml file
- gga_preprocessor.config = utilities.parse_config(args.config)
-
- # Transfer manual annotations
- gga_preprocessor.transfer_manual_annotations()
-
- # Transfer hectar annotations
- gga_preprocessor.transfer_hectar_annotations()
-
-
- # Format fasta headers
- gga_preprocessor.batch_modify_fasta_headers()
-
-
- # Clean up src_data
- gga_preprocessor.clean_up_dir_tree()
-
- logging.info("Datasets processed for %s" % gga_preprocessor.full_name)