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#!/usr/bin/python
# -*- coding: utf-8 -*-
import bioblend
import bioblend.galaxy.objects
from bioblend import galaxy
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import argparse
import os
import subprocess
import logging
import sys
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import yaml
import re
from datetime import datetime
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"""
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Usage: $ python3 deploy_stacks.py -i example.yml [OPTIONS]
"""
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def parse_input(input_file):
"""
Parse the yml input file to extract data to create the SpeciesData objects
Return a list of dictionaries. Each dictionary contains data tied to a species
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:param input_file:
:return:
"""
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parsed_sp_dict_list = []
if str(input_file).endswith("yml") or str(input_file).endswith("yaml"):
logging.debug("Input format used: YAML")
else:
logging.critical("Error, please input a YAML file")
sys.exit()
with open(input_file, 'r') as stream:
try:
yaml_dict = yaml.safe_load(stream)
for k, v in yaml_dict.items():
if k == "config":
pass
parsed_sp_dict_list.append(v)
except yaml.YAMLError as exit_code:
logging.critical(exit_code + " (YAML input file might be incorrect)")
sys.exit()
return parsed_sp_dict_list
class LoadData:
"""
Load data from the src_data subfolders into the galaxy instance's history of a given species
"""
def __init__(self, parameters_dictionary):
self.parameters_dictionary = parameters_dictionary
self.species = parameters_dictionary["description"]["species"]
self.genus = parameters_dictionary["description"]["genus"]
self.strain = parameters_dictionary["description"]["strain"]
self.sex = parameters_dictionary["description"]["sex"]
self.common = parameters_dictionary["description"]["common_name"]
self.date = datetime.today().strftime("%Y-%m-%d")
self.origin = parameters_dictionary["description"]["origin"]
self.performed = parameters_dictionary["data"]["performed_by"]
if parameters_dictionary["data"]["genome_version"] == "":
self.genome_version = "1.0"
else:
self.genome_version = parameters_dictionary["data"]["genome_version"]
if parameters_dictionary["data"]["ogs_version"] == "":
self.ogs_version = "1.0"
else:
self.ogs_version = parameters_dictionary["data"]["ogs_version"]
self.genus_lowercase = self.genus[0].lower() + self.genus[1:]
self.genus_uppercase = self.genus[0].upper() + self.genus[1:]
self.species_folder_name = "_".join([self.genus_lowercase, self.species, self.strain, self.sex])
self.full_name = " ".join([self.genus_uppercase, self.species, self.strain, self.sex])
self.abbreviation = " ".join([self.genus_lowercase[0], self.species, self.strain, self.sex])
self.genus_species = self.genus_lowercase + "_" + self.species
self.instance_url = "http://scratchgmodv1:8888/sp/" + self.genus_lowercase + "_" + self.species + "/galaxy/"
# Testing with localhost/scratchgmodv1
self.instance = None
self.history_id = None
self.library_id = None
self.script_dir = os.path.dirname(os.path.realpath(sys.argv[0]))
self.main_dir = None
self.species_dir = None
self.org_id = None
self.genome_analysis_id = None
self.ogs_analysis_id = None
self.tool_panel = None
self.datasets = dict()
self.source_files = dict()
self.workflow_name = None
self.metadata = dict()
self.api_key = "master" # TODO: set the key in config file --> saved for later (master api key access actions are limited)
if parameters_dictionary["data"]["parent_directory"] == "" or parameters_dictionary["data"]["parent_directory"] == "/path/to/closest/parent/dir":
self.source_data_dir = "/projet/sbr/phaeoexplorer/" # Testing path for phaeoexplorer data
else:
self.source_data_dir = parameters_dictionary["data"]["parent_directory"]
# Directory/subdirectories where data files are located (fasta, gff, ...)
self.do_update = False
# Update the instance (in histories corresponding to the input) instead of creating a new one
self.api_key = "master"
# API key used to communicate with the galaxy instance. Cannot be used to do user-tied actions
self.species_name_regex_litteral = "(?=\w*V)(?=\w*A)(?=\w*R)(?=\w*I)(?=\w*A)(?=\w*B)(?=\w*L)(?=\w*E)\w+" # Placeholder re
def modify_fasta_headers(self):
"""
Change the fasta headers before integration.
:return:
"""
try:
os.chdir(self.species_dir)
working_dir = os.getcwd()
except OSError:
logging.info("Cannot access " + self.species_dir + ", run with higher privileges")
logging.info("Fatal error: exit")
sys.exit()
self.source_files = dict()
annotation_dir, genome_dir = None, None
for d in [i[0] for i in os.walk(os.getcwd() + "/src_data")]:
if "annotation/" in d:
annotation_dir = d
for f in os.listdir(d):
if f.endswith("proteins.fasta"):
self.source_files["proteins_file"] = os.path.join(d, f)
elif f.endswith("transcripts-gff.fa"):
self.source_files["transcripts_file"] = os.path.join(d, f)
elif f.endswith(".gff"):
self.source_files["gff_file"] = os.path.join(d, f)
elif "genome/" in d:
genome_dir = d
for f in os.listdir(d):
if f.endswith(".fa"):
self.source_files["genome_file"] = os.path.join(d, f)
logging.debug("source files found:")
for k, v in self.source_files.items():
logging.debug("\t" + k + "\t" + v)
# Changing headers in the *proteins.fasta file from >mRNA* to >protein*
# production version
modify_pep_headers = [str(self.main_dir) + "/gga_load_data/utils/phaeoexplorer-change_pep_fasta_header.sh",
self.source_files["proteins_file"]]
# test version
# modify_pep_headers = ["/home/alebars/gga/phaeoexplorer-change_pep_fasta_header.sh",
# self.source_files["proteins_file"]]
logging.info("Changing fasta headers: " + self.source_files["proteins_file"])
subprocess.run(modify_pep_headers, stdout=subprocess.PIPE, cwd=annotation_dir)
# production version
modify_pep_headers = [str(self.main_dir) + "/gga_load_data/utils/phaeoexplorer-change_transcript_fasta_header.sh",
self.source_files["proteins_file"]]
# test version
# modify_pep_headers = ["/home/alebars/gga/phaeoexplorer-change_transcript_fasta_header.sh",
# self.source_files["proteins_file"]]
logging.info("Changing fasta headers: " + self.source_files["transcripts_file"])
subprocess.run(modify_pep_headers, stdout=subprocess.PIPE, cwd=annotation_dir)
# src_data cleaning
if os.path.exists(annotation_dir + "outfile"):
subprocess.run(["mv", annotation_dir + "/outfile", self.source_files["proteins_file"]],
stdout=subprocess.PIPE,
cwd=annotation_dir)
if os.path.exists(annotation_dir + "gmon.out"):
subprocess.run(["rm", annotation_dir + "/gmon.out"],
stdout=subprocess.PIPE,
cwd=annotation_dir)
def setup_data_libraries(self):
"""
- generate blast banks and docker-compose
- load data into the galaxy container with the galaxy_data_libs_SI.py script
:return:
"""
try:
logging.info("Loading data into the galaxy container")
subprocess.run("../serexec genus_species_galaxy /tool_deps/_conda/bin/python /opt/galaxy_data_libs_SI.py", shell=True)
except subprocess.CalledProcessError:
logging.info("Cannot load data into the galaxy container for " + self.full_name)
pass
else:
logging.info("Data successfully loaded into the galaxy container for " + self.full_name)
self.get_species_history_id()
# self.get_instance_attributes()
#
# # import all datasets into current history
# self.instance.histories.upload_dataset_from_library(history_id=self.history_id, lib_dataset_id=self.datasets["genome_file"])
# self.instance.histories.upload_dataset_from_library(history_id=self.history_id, lib_dataset_id=self.datasets["gff_file"])
# self.instance.histories.upload_dataset_from_library(history_id=self.history_id, lib_dataset_id=self.datasets["transcripts_file"])
# self.instance.histories.upload_dataset_from_library(history_id=self.history_id, lib_dataset_id=self.datasets["proteins_file"])
def generate_blast_banks(self):
"""
Automatically generate blast banks for a species and commit
:return:
"""
def connect_to_instance(self):
"""
Test the connection to the galaxy instance for the current organism
Exit if it cannot connect to the instance
"""
self.instance = galaxy.GalaxyInstance(url=self.instance_url, email="gga@sb-roscoff.fr", password="password",
verify=False)
logging.info("Connecting to the galaxy instance ...")
try:
self.instance.histories.get_histories()
self.tool_panel = self.instance.tools.get_tool_panel()
except bioblend.ConnectionError:
logging.critical("Cannot connect to galaxy instance @ " + self.instance_url)
sys.exit()
else:
logging.info("Successfully connected to galaxy instance @ " + self.instance_url)
self.instance.histories.create_history(name="FOO")
if __name__ == "__main__":
parser = argparse.ArgumentParser(description="Automatic data loading in containers and interaction "
"with galaxy instances for GGA"
", following the protocol @ "
"http://gitlab.sb-roscoff.fr/abims/e-infra/gga")
parser.add_argument("input",
type=str,
help="Input file (yml)")
parser.add_argument("-v", "--verbose",
help="Increase output verbosity",
action="store_false")
args = parser.parse_args()
if args.verbose:
logging.basicConfig(level=logging.DEBUG)
else:
logging.basicConfig(level=logging.INFO)
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logging.info("Load data: start")
sp_dict_list = parse_input(args.input)
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for sp_dict in sp_dict_list:
o = LoadData(parameters_dictionary=sp_dict)
o.main_dir = os.path.abspath(args.dir)
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o.modify_fasta_headers()
logging.info("Successfully formatted files headers " + o.genus[0].upper() + ". " + o.species + " " + o.strain + " " + o.sex)
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# o.setup_data_libraries()
# logging.info("Successfully set up data libraries in galaxy for " + o.genus[0].upper() + ". " + o.species + " " + o.strain + " " + o.sex)
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logging.info("Load data: done")